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Assay Development: Fundamentals and Practices - download pdf or read online

By Ge Wu

ISBN-10: 0470191155

ISBN-13: 9780470191156

ISBN-10: 0470583118

ISBN-13: 9780470583111

Essential ideas and perform of assay development

The first entire, built-in remedy of the topic, Assay improvement: basics and Practices covers the necessities and strategies concerned about undertaking an assay undertaking in both a biotechnology/drug discovery atmosphere or a platform atmosphere.

Rather than trying accomplished insurance of all assay improvement applied sciences, the ebook introduces the main typical assay improvement applied sciences and illustrates the paintings of assay improvement via a couple of quite often encountered organic ambitions in assay improvement (e.g., proteases, kinases, ion channels, and G protein-coupled receptors). barely enough organic historical past for those organic goals is equipped in order that the reader can persist with the logics of assay improvement. Chapters discuss:

  • The fundamentals of assay improvement, together with foundational ideas and applications

  • Commonly used instrumental tools for either biochemical assays and cell-based assays

  • Assay innovations for protein binding and enzymatic activity

  • Cell-based assays

  • High-throughput screening

An in-depth examine of the now renowned Caliper's off-chip kinase assay presents an instructive, real-world instance of the assay improvement strategy.

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Extra resources for Assay Development: Fundamentals and Practices

Example text

GLP is applied in safety assessment of a drug’s pharmacology, pharmacokinetics, and toxicity in animal studies. GCP is applied in all three phases of clinical studies to protect human subjects. GMP is applied to both the manufacturing of the reagents used in clinical testing and the manufacturing of the final marketed product after the approval of the drug. of the reagents used in clinical testing and the manufacturing of the final marketed product after the approval of the drug. During the preclinical development phase the drug candidate’s initial safety and activity profile is investigated to support the investigational new drug (IND) application filing.

And Whitten, D. G. (2007) Design considerations for high-throughput screening and in vitro diagnostic assays. Comb. Chem. High Throughput Screen. 10, 399–412. Bleicher, K. , and Alanine, A. I. (2003) Hit and lead generation: Beyond highthroughput screening. Nat. Rev. Drug Discov. 2, 369– 378. Burtis, C. , Ashwood, E. , and Bruns, D. E. (2007) Tietz Fundamentals of Clinical Chemistry, 6th ed. Saunders, New York. Chorghade, M. S. ) (2006) Drug Discovery and Development, Drug Discovery, Vol. 1. Wiley, Hoboken, NJ.

11. , pH, temperature, buffer components and their concentration, enzyme concentration, substrate concentration, cell number). 12. Reproducibility The ability to carry out the assay and obtain specified results with a combination of any of the following: a different scientist, a different time, a different location, a different batch of assay components, and different instruments, etc. 13. Scalability The ability of the assay to perform in different formats (test tubes, different microtiter plates: 12-well, 24-well, 48-well, 96-well, 384-well, 1536well) and in different scales (screen a few sample per run vs.

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Assay Development: Fundamentals and Practices by Ge Wu


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